Diagnosis of Avian Tuberculosis in laying hens by pathological, microbiological and polymerase chain reaction (PCR): Case report

Avian tuberculosis was described at 50 week-old Lohmann chickens in a commercial chicken farm. The study materials were 20 pieces of from 2 coops and 16 pullets 4 After necropsy, samples processed routinely for histopathological microbiological examinations. 
 Macroscopically, hard consistency, numerous different sizes, whitish-yellow caseificated-calcifiated nodules seen on the liver, spleen, kidneys intestinal serosa. At microscopic examination, various sized granulomas, which have been caseification necrosis surrounded by epithelioid histiocytes multinucleated giant cells, found wall serosa intestine. Numerous acid-fast bacteria histopathology macrophages spleen intestines Ziehl – Neelsen staining. Mycobacterium avium spp produced inoculations ovaries. subsp. these cultures identified PCR. It thought that infection could be fecal-oral route due to both hens common disease coop. Therefore, role manure may also taken into account spreading disease.